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CRISPR-Cas: A Laboratory Manual. Jennifer Doudna

CRISPR-Cas-A-Laboratory-Manual.pdf
ISBN: 9781621821311 | 450 pages | 12 Mb

• CRISPR-Cas: A Laboratory Manual
• Jennifer Doudna
• Page: 450
• Format: pdf, ePub, fb2, mobi
• ISBN: 9781621821311
• Publisher: Cold Spring Harbor Laboratory Press

Download CRISPR-Cas: A Laboratory Manual

Download bestseller ebooks free CRISPR-Cas: A Laboratory Manual 9781621821311

CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease
CRISPR/Cas9-derived RNA-guided nucleases (RGNs) are DNA A Laboratory Manual, 3rd Ed. Sambrook, J. F. & Russell, D. W. (eds.)  Crispr/Cas: A Laboratory Manual von University Jennifer Doudna
Crispr/Cas: A Laboratory Manual von University Jennifer Doudna, Prashant Mali ( ISBN 978-1-62182-130-4) versandkostenfrei vorbestellen. Lieferung direkt  Misty River Books - BookManager
Cover image for Crispr/Cas · More Info · Crispr/Cas: A Laboratory Manual | Paperback Jennifer Doudna | Prashant Mali Cold Spring Harbor Laboratory Press Guide-it™ CRISPR/Cas9 Systems User Manual - Clontech
Clontech Laboratories, Inc. Guide-it™. CRISPR/Cas9 Systems. User Manual. Cat . No(s). 632601, 632602. (033015). Clontech Laboratories, Inc. A Takara Bio  RNA-Guided Genome Editing of Mammalian Cells. - Zhang Lab @ MIT
Key words CRISPR-Cas Genome editing DNA cleavage Cas9 Guide RNA PAM sequence NHEJ provided in the SURVEYOR Mutation Detection Kit manual.

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